Effects of Long Non-coding Ribonucleic Acid Plasmacytoma Variant Translocation 1 Regulating Octamer Binding Transcription Factor 4 Expression on Hepatocellular Carcinoma Cells
نویسندگان
چکیده
The researchers aimed to evaluate the effects of long non-coding ribonucleic acid plasmacytoma variant translocation 1 (lncRNA PVT1) regulating octamer binding transcription factor 4 (OCT4) expression on hepatocellular carcinoma (HCC) cells. It was discovered that in contrast blank group, si-PVT1 group had lower PVT1 and OCT4 expressions, number invading cells wound healing rate, higher radiosensitivity (P<0.05). served as a gene targeted by lncRNA PVT1. exhibited declined luciferase activity compared with NC after transfection wild-type In comparison those sh-NC rate dropped sh-OCT4 At same radiation dose, survival fraction than observed Silencing significantly suppresses HCC cell invasion well migration elevates their radiosensitivity, probably down-regulating expression.
منابع مشابه
Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4
The present study aimed to investigate the effects of lentiviral infection of human umbilical cord mesenchymal stem cells (hUCMSCs) on the expression of octamer transcription factor 4 (Oct4). hUCMSCs were infected with lentivirus carrying the green fluorescent protein gene (GFP) at different multiplicities of infection (MOI), and the optimal MOI was determined by flow cytometry; the proliferati...
متن کاملDysregulated Expression of Long Intergenic Non-coding RNAs (LincRNAs) in Urothelial Bladder Carcinoma
Long intergenic non-coding RNA (lincRNA) has been introduced as key regulators of diverse biological processes, including transcription, chromatin organization, cell growth and tumorigenesis. With regard to the potential role of lincRNAs in cancer development, one may postulate that differential expression of lincRNAs could be employed as a tool in cancer diagnosis, prognosis, and targeted ther...
متن کاملHigh expression of octamer transcription factor 1 in cervical cancer
Cervical carcinoma is the second most prevalent malignancy in females worldwide. The crucial etiologic factors involved in the development of cervical carcinoma include infection with papillomavirus, and the structural or functional mutation of oncogenes and tumor suppressor genes. The abnormal change of octamer transcription factor 1 (OCT1) is associated with tumor progression and a poor patie...
متن کاملHomocysteine Induces Heme Oxygenase-1 Expression via Transcription Factor Nrf2 Activation in HepG2 Cells
Background: Elevated level of plasma homocysteine has been related to various diseases. Patients with hyperhomocysteinemia can develop hepatic steatosis and fibrosis. We hypothesized that oxidative stress induced by homocysteine might play an important role in pathogenesis of liver injury. Also, the cellular response designed to combat oxidative stress is primarily controlled by the transcripti...
متن کاملCell cycle and pluripotency: Convergence on octamer-binding transcription factor 4
Embryonic stem cells (ESCs) have unlimited expansion potential and the ability to differentiate into all somatic cell types for regenerative medicine and disease model studies. Octamer‑binding transcription factor 4 (OCT4), encoded by the POU domain, class 5, transcription factor 1 gene, is a transcription factor vital for maintaining ESC pluripotency and somatic reprogramming. Many studies hav...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Human Genetics
سال: 2022
ISSN: ['0972-3757', '2456-6330']
DOI: https://doi.org/10.31901/24566330.2022/22.04.832